CRISPR plasmid construction, $99/construct
gRNA design, synthesis and cloning into any vector of your choice. Customer provides gene name and species or NCBI accession number. We deliver 4 g mini-prep plasmid DNA and sequencing chromatogram.
Donor plasmid construction, from $0.19/bp
Knockin donor sequence design, synthesis and cloning into any vector of your choice.
Plasmids for in vitro transcription, $99/construct Plasmid contains T7 promoter and your crRNA and tracrRNA sequences.
Custom plasmids
Design your own plasmid, e.g change a promoter and have us to make it for you.
Custom mRNAs
mRNA generated from in vitro transcription, capped and polyA –tailed if required. Customer may use our plasmid service to construct a plasmid or provide us with a plasmid.
GENE SYNTHESIS
Top Gene Technology gene synthesis services provide solutions to challenges you may face in testing, sub cloning, creating fusion proteins, or achieving sufficiently high protein expression.
MUTAGENESIS
Top Gene Technologies can increase your research efficiency by performing site-directed mutagenesis. We can generate your desired insertions, deletions, or point mutations.
CRISPR SERVICES
CRISPR plasmid construction, $99/construct, gRNA design, synthesis and cloning into any vector of your choice.
KNOCKOUT YEAST
Gene knockout/in yeast strains are good tools for functional genomic studies.
SUBCLONING
Top Gene Technologies services in gene cloning and subcloning, plasmid construction, and fusion gene construction.
PROTEIN EXPRESSION
Expression constructs can be transfected into any of a variety of E. coli strains and clones screened in small-scale pilot experiments to determine the best induction and expression conditions.
